Confocal and super-resolution microscopes

• Nikon AXR point scanning confocal with NSPARC detector 
• Leica Stellaris 5 point scanning confocal with white-light laser “Bo-Peep”
• Andor Dragonfly 602 spinning disk confocal microscope
• Leica SP8 point scanning confocal with white-light, STED and multiphoton lasers, “Buzz”
• Leica SP8 point scanning confocal with diode lasers and digital light sheet, “Woody”
• Leica SR GSD 3D TIRF and STORM

Widefield microscopes

• Leica DM5500B upright microscope
• Nikon Ti2E microscope, “Bernie”
• Leica-Eppendorf microinjection system

Stereomicrocopes

• Leica Thunder Stereomicroscopy System
• Leica M205 FA stereomicroscope
• Leica Ivesta 3 stereomicroscope

Laser-capture microdissection

• ArcturusXT Laser Capture Microdissection System

Image analysis and processing

• Huygens Professional: rapid 2D and 3D deconvolution of confocal, STED and widefield images
   
• Imaris: 3D reconstruction of optical sections, measurements in 3D, isosurfacing, animations, colocalization analysis.
   
• Leica LAS X 3D analysis and measurements: 3D multichannel analysis, measurements and object classification
   
• Fiji/ImageJ: image processing and analysis
   
• Nikon Elements

Sample preparation

• Leica CM3050S cryostat for BSL-1 samples for cutting 0.5-300 micron-thick tissue sections of frozen samples
   
• Leica CM3050S cryostat for BSL-2 samples
   
• Leica CM1950 cryostat for cutting Biosafety Level 2 1-100 µm-thick frozen tissue samples
   
• Leica ARTOS 3D ultramicrotome for cutting semi-thin and thin sections of resin-embedded samples for light and electron microscopy
   
• Paraffin microtome for cutting 1-50 micron thick paraffin sections
   
• Vibratome 3000 Plus for cutting variable thicknesses as thin as 100 µm of live or fixed specimens with a maximum specimen size of 60x40x15mm

Supply center

ALMC has an on-site supply center stocked with microscopy reagents including slides, cover slips, mounting media, imaging chamber slides and some dyes. Consumables for laser capture microdissection are also available.

File transfer system

ALMC users are responsible for storing and backing up their own data. The ALMC provides access to the Research Data Ecosystem (RDE) from all computers at our facility. We encourage users to save data directly to their lab’s RDE folder. The ALMC also provides a TEMPORARY folder within its RDE storage to facilitate the transfer of data generated at the core. Due to space limitations, all files in this folder are deleted at 8 a.m. on the first day of every month. Users are responsible for transferring their files to their own storage before this deadline. For instructions on connecting to the ALMC RDE, contact ALMicroscopy@missouri.edu. For general questions about the MU RDE, contact itrss@missouri.edu or your department’s IT professional. Users who do not bring their own storage device and choose not to use the RDE will be charged $20 for a portable USB storage device.