Leica TCS SP8 with digital light-sheet and diode lasers

The Leica TCS SP8 system possesses fixed diode laser lines (405, 488, 552, 638 nm) that can be utilized for conventional confocal microscopy or digital light-sheet applications. Illumination with the light sheet allows rapid acquisition of multiple images (>100 frames/second) to permit 3-D rendering and results in low phototoxicity. Imaging can be performed for entire organs (e.g., Arabidopsis roots) or organisms (e.g., Drosophila embryos, developing zebra fish). Extended time-lapse acquisition can be used to monitor physiological or developmental changes over time.

Download a PDF describing the basics of light-sheet microscopy and providing protocols for sample preparation.

The following objectives are available for use with the confocal and light-sheet systems:

Mag.Objective descriptionNAImmersion mediumWorking distance (mm)
10xHC PL APO CS0.40Air2.20
20xHC PL APO CS0.70Air0.59
20xHC PL APO IMM CORR CS20.75Water, glycerol, or oil0.68
25xHCX IRAPO L0.95Water2.40
40xHC PL APO CORR CS21.10Water0.62
40xHC PL APO CS21.30Oil0.24
63xHC PL APO CS21.40Oil0.14
63xHC PL APO CORR CS21.30Glycerol0.30
63x HC PL APO CORR CS21.20Water0.30
100xHCX PL APO CORR CS1.44Oil0.10
100xHC PL APO STED WHITE1.40Oil0.13

 

The following objectives are specifically for use with the light-sheet system:

Mag.Objective descriptionNAImmersion mediumWorking distance (mm)
2.5xHC PL FLUOTAR0.07Air9.4
10xHC APO DLS L0.30Water3.6
25xHC PL APO IMM CORR CS20.75Water2.5