Image
Zeiss Axiovert 200M with fluorescence and ORCA-ER camera

The Zeiss Axiovert 200M inverted microscope is a fully automated instrument with a motorized stage and z-drive. A featured technique on this system is fluorescent microscopy; however, the system is also capable of transmitted light bright-field. In addition, the microscope can acquire high-quality composite images of whole-tissue slices (a process termed "tiling") for both single- and multi-channel fluorescence. A wide range of objectives and fluorescence filter sets are available. Images are acquired by a Hamamatsu ORCA-ER CCD camera, a high sensitivity, low-noise device for high resolution imaging. An environmental chamber with temperature and CO2 control is available for extended imaging of live cells. The system operates under the control of the MetaMorph v.7.8.12 software program. This program enables complex experimental designs, including time-lapse imaging, autofocusing, streaming and z-stack acquisition.

The following objectives are available for this system:

Mag.Objective descriptionNAImmersion mediumWorking distance (mm)Pixel size (um)Field of view
5xPlan Apochromat0.16Air12.1  
10xEC Plan-NeoFluar0.3Air5.60.99581338.4 x 1019.7
20xPlan-Apochromat0.75Air0.61  
20xPlan-Apochromat0.8Air0.550.4959666.4 x 507.8
25xLCI Plan-NeoFluar0.8Water/glycerol0.210.3947530.5 x 404.2
40xLD Plan-NeoFluar0.6Air2.5-3.30.2490334.7 x 255.0
40xEC Plan-NeoFluar1.3Oil0.210.2490334.7 x 255.0
40xC-Apochromat1.2Water0.29  
40xC-Apochromat1.2Water0.280.2454330.0 x 251.3
63xPlan-Apochromat1.4Oil0.180.1569210.9 x 160.7
63xPlan-Apochromat DIC1.4Oil0.180.1569210.9 x 160.7
63xC-Apochromat1.2Water0.280.1576211.8 x 161.4

 

The following fluorescent filters are available for this microscope:

FluorFilter setExcitationEmission
DAPI49000325-375435-485
DAPI LPFS2365LP420
CFPFS 47426-446460-500
eGFP/FITC49002450-490500-550
YFPFS 46490-510520-550
Cy3/TRITC49004532-557570-640
DSRed49005530-560590-650
Cy549006590-650662-737
Cy749007642-747765-855