The Zeiss Axiovert 200M inverted microscope is a fully automated instrument with a motorized stage and z-drive. This microscope is capable of brightfield and DIC. A featured technique on this system is slide scanning (the automated acquisition of images across a defined region), with the possibility of acquiring high-quality composite images from whole-tissue slides. A wide range of low and high magnification objectives are available. The selection of cameras includes a Leica DFC290 color camera. An environmental chamber with temperature and CO2 control is available for extended imaging of live cells. The system operates under control of the MetaMorph software v.7.8.12.
The following objectives are available for this system:
| Mag. | Objective Description | NA | Immersion Medium | Working distance (mm) | Application |
|---|---|---|---|---|---|
| 5x | Plan Apochromat | 0.16 | Air | 12.1 | BF |
| 10x | EC Plan-NeoFluar | 0.3 | Air | 5.6 | BF, DIC II |
| 20x | Plan-Apochromat | 0.75 | Air | 0.61 | BF, DIC II |
| 20x | Plan-Apochromat | 0.8 | Air | 0.55 | BF, DIC II |
| 25x | LCI Plan-NeoFluar | 0.8 | Water/glycerol | 0.21 | BF |
| 40x | LD Plan-NeoFluar | 0.6 | Air | 2.5-3.3 | BF |
| 40x | EC Plan-NeoFluar | 1.3 | Oil | 0.21 | BF, DIC III |
| 40x | C-Apochromat | 1.2 | Water | 0.29 | BF, DIC III |
| 40x | C-Apochromat | 1.2 | Water | 0.28 | BF, DIC III |
| 63x | Plan-Apochromat | 1.4 | Oil | 0.18 | BF, DIC III |
| 63x | Plan-Apochromat DIC | 1.4 | Oil | 0.18 | BF, DIC III |
| 63x | C-Apochromat | 1.2 | Water | 0.28 | BF |
BF-bright field, DIC-differential interference contrast