An inverted, fully automated Leica DMI4000 B with integrated Eppendorf Microinjection system and a 1.4 megapixel, 12-bit, cooled CCD camera from Q-Imaging. This system is capable of injecting a single cell with volumes as small as a femtoliter. The capture software from Q-Imaging allows for quick and easy collection of images for traditional microscopy applications as well.
This system is equipped with four objective lenses:
| Mag. | Objective Description | NA | Immersion Medium | μm per pixel | Field of View (μm) | Working Distance(mm) | Applications* |
|---|---|---|---|---|---|---|---|
| 5x | N Plan | 0.12 | Air | 1.85874 | 2587.36 x 1933.09 | 14 | BF, FL, Ph0, DIC |
| 10x | N Plan | 0.25 | Air | 0.945017 | 1315.46 x 982.82 | 5.8 | BF, FL, Ph1, DIC |
| 20x | N Plan Corr | 0.4 | Air | 0.464134 | 646.07 x 482.69 | 3.2-1.9 | BF, FL, Ph1, DIC |
| 40x | HCX PL Fluotar L Corr | 0.6 | Air | 0.229084 | 318.88 x 238.25 | 3.3-1.9 | BF, FL, Ph2, DIC |
The following filters are available on this system:
DAPI (Leica A4)
Texas Red (Leica N3)
GFP (Leica L5)
The following are example images taken by MU researchers:
Diatomes imaged using DIC (Differential Interference Contrast) for enhanced details.
Diatomes imaged using Phase Contrast, a technique different than DIC, but also for enhancing certain details.
Image of a cell being injected.
Image of nuclear transfer in an oocyte. Taken by Ye Yuan.