The Leica M205 FA stereo microscope is a fully automated instrument with a motorized z-drive, focus, zoom and filter wheel controls for multichannel time-lapse experiments. The available filter sets include DAPI, CFP, GFP, YFP, and dsRed. The featured objective has a zoom range of 20.5:1 and a working distance of 61.5 mm. The system includes a transmitted light base as well as an O-ring LED illuminator with 48 LEDs. Images are captured with a 2.8 Mpixel Leica DFC7000T color, high-sensitivity, cooled digital camera.
Some special features include Automontage software for extended depth of focus imaging and Rotterman's relief contrast to visualize unstained transparent objects. The system also features a PhotoFluor II illuminator which is an adjustable fluorescence light source providing a very stable high-power output essential for time-lapse and quantitative fluorescent imaging.
(Montage image above taken by Michele Warmund. Right image is the multifocus image, and left image is one of the single images in the stack.)
The following fluorescent filters are available for the Leica M205 FA stereo microscope:
UV
CFP
GFP
YFP
dsRed
More example montage images (Michele Warmund)
