Image
The Leica GSD 3D super-resolution microscope with TIRF possesses 4 different laser lines (488, 560, 642 and 405 nm) and a fast, highly sensitive Andor iXon Ultra 897 EMCCD camera for detecting up to 3 fluorophores. The microscope also includes a fully automated TIRF system, allowing super-resolution to be combined with TIRF microscopy.
Download a PDF document describing the basics of GSD microscopy and providing protocols for sample preparation.
The following objectives are available for use with the GSD system:
| Mag. | Objective Description | NA | Immersion Medium | Working distance (mm) |
|---|---|---|---|---|
| 10x | HCX PL FLUOTAR | 0.30 | Air | 11 |
| 100x | HC PL APO CORR TIRF | 1.47 | Oil | 0.09 |
| 160x | HC PL APO | 1.43 | Oil | 0.07 |