ArcturusXT Laser Capture Microdissection System

The ArturusXT system is designed for UV laser cutting (microdissection) and IR laser capture of individual cells and cell clusters from a wide variety of tissue samples. The system is mounted on an automated Nikon Eclipse TE2000 inverted microscope capable of brightfield, phase-contrast, DIC, and fluorescence microscopy and equipped with a color CCD camera.

This system is equipped with five objective lenses

The following fluorescence filters are available on this system (excitation, emission)

340-390, >410 (e.g. DAPI, Hoechst)

455-495, >510 (e.g. FITC, GFPs, Alexa 488)

503-548, >565 (e.g. Cy3, rhodamine, RFPs, Alexa 546)

570-630, >655 (e.g. Cy5, Alexa 633, Alexa 647)

Technology resources

• Laser capture microdissection (LCM) resources on the ThermoFisher website

• Arcturus® LCM process overview

• LCM kits and reagents

Application notes and protocols

• An approach for analyzing genetic heterogeneity in retrospective tumor samples using laser capture microdissection, real-time PCR, and next-generation sequencing (PDF)

• Gene expression profiling of a single cell using laser capture microdissection and OpenArray® technology (PDF)

• Optimized isolation of live plant tissue using laser microdissection for use in gene expression analysis (PDF)

• Laser capture microdissection of cells from plant tissues

• Laser capture microdissection protocol for xylem tissues of woody plants

• Development of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots