ALMC is a campuswide resource for light microscopy, immunocytochemistry and in situ hybridization techniques. The instruments and equipment in the ALMC are available for all to use on a per hour billing schedule. We encourage users to become proficient enough to use the instruments independently, so most users go through an initial training session. The length of the training session depends on the instrument, application and level of user experience; times below are estimates for basic training of an inexperienced user. In addition, we offer assisted use during which core staff will operate the instrument. All assisted use and training is $30/hour plus the base usage fee for the instrument. 

The core is also open to academic and commercial users outside of the University of Missouri at different rate plans; please call 573-882-4895 for more information. 

Leica TCP SP8 STED and MP

Confocal microscope with tunable white light laser (470-670 nm), fixed visible laser lines (405, 458, 476, 488, 496, 514 nm), Mai Tai DeepSee multiphoton laser tunable from 680-1060 nm, and STED super-resolution system with three depletion laser lines (592, 660, and 775). 

Service Fee
Base usage fee for regular confocal (tunable white light and fixed visible laser lines) $35.00/hr
Initial training (~2 hr) or assisted use for regular confocal $65.00/hr
Usage fee for the multiphoton laser and FLIM equipment $40.00/hr
Training or assisted use for the multiphoton laser $70.00/hr
Usage fee for the STED system $35.00/hr
Training or assisted use for the STED system $65.00/hr

 

Leica TCP SP8 with digital light-sheet and diode lasers

Confocal microscope with fixed diode laser lines (405, 488, 552, and 638 nm) and digital light-sheet module with a Hamamatsu Orca-Flash4.0 Digital CMOS camera.

Service Fee
Base usage fee for regular confocal (diode lasers) or digital light-sheet module by trained clients $30.00/hr
Initial training (~2 hr) or assisted use $60.00/hr

 

Leica GSD 3D 

Wide-field super-resolution microscope with 405, 488, 560 and 642 nm lasers and Andor iXon Ultra 897 EMCCD camera.

Service Fee
Base usage fee for trained clients $20.00/hr
Initial training (~2 hr) or assisted use $50.00/hr

 

Zeiss Axiovert 200M inverted microscope with color digital camera

Motorized stage and Z-drive, brightfield and differential interference contrast (DIC). Capable of slide scanning for tiling images into a composite image.

Service Fee
Base usage fee for trained clients $5.00/hr
Initial training or assisted use $35.00/hr

 

Zeiss Axiovert 200M inverted microscope with fluorescence and ORCA-ER camera 

Motorized stage and Z-drive, brightfield and florescence. Capable of slide scanning for tiling images into a composite image.

Service Fee
Base usage fee for trained clients $5.00/hr
Initial training or assisted use $35.00/hr

 

Leica DM 5500B upright microscope with color digital camera 

Versatile microscope capable of brightfield and DIC. Image acquisition performed with a high resolution Leica DFC290 color digital camera and user-friendly LAS software. 

Service Fee
Base usage fee for trained clients $5.00/hr
Initial training (~30 min) or assisted use $35.00/hr

 

Leica M205 FA fully motorized stereomicroscope with z-drive 

Equipped with transmitted, reflected and epifluorescence illumination sources. Images are acquired with the Leica DFC7000T color, high-sensitivity, cooled digital camera 

Service Fee
Base usage fee for trained clients $5.00/hr
Initial training (~30 min) or assisted use $35.00/hr

 

Leica MZ FLIII Stereoscope 

Optronics CCD camera, epifluorescence and brightfield with Magnafire acquisition software

Service Fee
Base usage fee for trained clients $2.00/hr
Initial training (~15 min) or assisted use $32.00/hr

 

Leica DMI4000 B with integrated Eppendorf microinjection system 

Inverted microscope with brightfield, epifluorescence and fully-automated DIC and phase-contrast. Image and movie capture with 1.4 megapixel 12-bit, cooled CCD camera from Q-Imaging. 

Service Fee
Base usage fee for trained clients $17.50/hr
Initial training (~30 min for microscope only) or assisted use $47.50/hr

 

Applied Biosystems® ArcturusXT laser capture microdissection system 

Microdissection/capture of tissue with UV and IR laser. Brightfield, phase contrast, DIC/Nomarski and epifluorescence microscopy with color CCD camera. 

Service Fee
Base usage fee for trained clients $40.00/hr
Initial training or assisted use $50.00/hr

 

Image Processing 

Huygens Professional, Metamorph, AutoQuant, Imaris, Fiji / ImageJ, Photoshop 

High-end computer workstations available for image processing/analysis and figure preparation including deconvolution for blur reduction, 3D rendering, movies/animation and analytical software for image-based measurements and morphometry.

Service Fee
Base usage fee for trained clients $5.00/hr
Initial training or assisted use $35.00/hr
Deconvolution batch processing (after hours) $10.00/evening

 

Tissue Sectioning 

Paraffin microtome

1-50 µm-thick paraffin-embedded sections 

Service Fee
Base usage fee for trained clients $2.00/hr
Initial training (duration depends on application, includes use fee) $32.00/hr
Blades $3.00 ea
De-waxing paraffin sections on slides Please call for pricing

 

Ultramicrotome 

Reichert Ultracut S generates 0.1-2.5 µm-thick plastic sections for light and electron microscopy 

Service Fee
Base fee for trained users $3.00/hr
Initial training (duration depends on application, includes use fee) $40.00/hr
Use of LKB glass knife maker (glass strips included) $11.50/strip

 

Sample Preparation 

Service Fee
Nunc 35 mm glass base imaging dishes (12 mm well), sterile $7.75/dish
MatTek 35 mm glass base imaging dishes (20 mm well) $6.40/dish
MOWIOL mounting medium (1.5 mL) $2.00
Cover slips-various sizes (#1.5 thickness) $13.00/box
Glass Slides $20.00/box
Superfrost Excell glass slides $32.00/box
Loctite 404 Instant Adhesive $32.00

 

LCM Consumables

Service Fee
CapSure Macro LCM Caps (48 per box) $276.00/box
PEN Membrane Glass Slides (50 slides) $270.00/box

 

Miscellaneous 

Service Fee
700 MB CD-R $1.00
4.7 GB DVD+R $1.00
Consultation with Core staff: detailed discussion of experimental design/execution, and/or demonstration or assistance (first hour free) $30.00

 

Life Technologies Supply Center

ALMC has an on-site Life Technologies Supply Center stocked with a wide variety of fluorescent markers. In addition to our normal inventory, clients may special order anything from the Life Technologies catalog without shipping fees.