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Bruker timsTOF Pro
- Hybrid trapped ion-mobility spectrometry, quadrupole, time-of-flight LC-MS+MS/MS
- Accurate mass, high resolution, instrument (routinely <10 parts per million, 50,000 resolution)
- High-coverage global quantitative proteomics
- PTM mapping and quantitation
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ThermoFisher Scientific LTQ Orbitrap XL
- Hybrid ion trap, Orbitrap mass spectrometer
- Accurate mass instrument (routinely < 5 ppm on precursor)
- High resolution, typically 30,000 for peptides
- Very high resolution possible (>100,000 at m/z 400)
- Reversed-phase separation of peptides using integrated Proxeon nano-HPLC
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Agilent 6520 Accurate Mass QTOF
- Hybrid quadrupole time-of-flight mass spectrometer
- Integrated nano-HPLC
- Integrated CHIP CUBE (microfluidic C18 column for peptide separation)
- Typical peptide resolution >10,000
- Typical peptide mass accuracy <5 ppm
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Amersham IPGphor & Bio-Rad Protean IEF
- Isoelectric focusing units
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Amersham SE 600, Ettan DALTsix, & SE62
- Second-dimension gel electrophoresis and electrophoretic transfer units
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Fujifilm FLA5000
- Laser imaging system (three lasers, dual photomultiplier tubes)
- Capable of imaging Cy2, Cy3, Cy5 dyes, Sypro, ProQ, etc. fluorescent stains
- Phosphoimaging capability (NO radioactive samples are allowed in the Center)
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Waters Xevo TQ-S
- Triple quadrupole mass spectrometer
- UHPLC with ESI or APCI source
- High sensitivity and quantitation of targeted compounds in both positive and negative ion modes
- Part of the Small Molecule MS Lab