Example of data

The Gehrke Proteomics Center accepts any non-hazardous, non-pathogenic, and non-radioactive samples for analyses. These samples can be whole organism (cell pellets, seedlings, etc.), tissue (biopsies, roots, leaves, etc.), or purified protein (cell lysate, precipitated protein, IPs, gel bands/spots). 

We have standard operating procedures for each type of sample submission, but we welcome discussion of the goals of the experiment prior to initiation of a project (please contact Dr. Brian Mooney at mooneyb@missouri.edu). 

Small Molecule MS Lab

The Small Molecule MS Lab is part of the Proteomics Center and is hosted by the Department of Biochemistry in Schweitzer Hall. We aim to assist campus researchers with small molecule analysis via liquid chromatography coupled to mass spectrometry (LC/MS). Our current instrumentation is used to quantify known compounds, for which standards are available. If you are interested in having experiments/analyses done in the lab, please contact the staff to discuss your project. More about the Small Molecule MS Lab.

Global quantitative proteomics by mass spectrometry 

LC-based separation of complex samples followed by simultaneous identification and quantitation of proteins is achieved using accurate mass instruments (Thermo Scientific LTQ Orbitrap or Agilent 6520 QTOF). The Center allows client-access to software for subsequent quantitative analyses, but Center staff can also perform these analyses as fee-for-service. (Example data shown above.)

Targeted quantitative proteomics by LC-MRM

LC-based separation of complex samples followed by targeted quantitation of specific peptides/proteins using the Xevo triple quadrupole (QQQ). The instrument is capable of sensitive quantitation of proteins (e.g. biomarkers) or peptides (e.g. phosphorylation site occupancy).

Mass spectrometry-based identification of proteins

Identification of proteins is performed by obtaining data on trypsin-digested peptides and comparing these against public databases using MASCOT or Sequest software. Increasingly complex samples are analyzed by liquid chromatography mass spectrometry on the LTQ Orbitrap or Agilent 6520 QTOF.

Intact mass analyses

Intact mass (molecular weight) of purified proteins are obtained using our Agilent 6520 QTOF high-mass accuracy instrument incorporates protein (C8 columns) LCMS for accurate mass intact protein analyses.

Post-translational modifications

Fluorescent stains and 2D gel electrophoresis allows specific imaging of phospho-proteins (ProQ-Diamond) and glyco-proteins (ProQ-Emerald). Additionally, post-translational modifications can be mapped by mass spectrometry. Mapping sites is not trivial, but the Center provides this service through extensive consultation with the client prior to, and on completion of, the analyses. 

Targeted small molecule analysis by mass spectrometry

UPLC based separation of samples followed by tandem mass spectrometry (MRM) for quantitation of small molecules (e.g. drugs) is available. A triple quadrupole (Waters XevoTQS) is used for these analyses and quantitative studies are possible with compound-matched standards. 

Two-dimensional electrophoresis

The Center performs high resolution large format (20x24cm) 2D-gel electrophoresis on any non-hazardous biological samples. The Center will accept whole organism, cell or tissue, or purified protein samples. The Center is also capable of performing Western blots. 

Image analysis

The Center can capture 16-bit TIFF images of 2D gels. Gels can be analyzed in-house (by staff or the client) using ImageMaster Platinum and/or DeCyder programs, the latter for analysis of DIGE (Cy dye-labeled) gels. 

Center staff support 

The Center emphasizes its full-service aspect by involving staff and investigators at all times during sample processing. We facilitate experimental design, data analysis, manuscript figure/methods preparation, grant application figures, methods, and facilities description preparation. Additionally, investigators can be trained by staff on sample prep, software, and even instrumentation if, for example, a project requires extensive mass spectrometric analyses.