Rates are for University of Missouri users. External users should contact Associate Director Brian Mooney.

Protein Identification 

Service Cost
Gel band or spot (coomassie-stained) $192 (digest + QTOF/Orbitrap LC-MS/MS)
20 bands/spots by LCMS/MS $2,860 (includes spot excision)
1 band/spot client-processed (with Center reagents) $97 (Digest + LC-MS/MS)
Protein in solution (e.g. recombinant protein or complex lysate) $96.70 (digest + large-format ZIP tip + Bruker timsTOF LC-MS/MS)


Note: this does not include determination of protein concentration (add $27 for this service)

 

High-Coverage Global Proteomics
Bruker timsTOF-PRO with PASEF1

Service Cost
Differential Protein Abundance analyses of a Biological Quintuplet2 $2,796 (Identification and Quantitation of >2000 proteins) 
$3,530 (Data-Independent Acquisition, ID & Quant of >3000 proteins)

Note 1: Parallel Accumulation-Serial Fragmentation (PASEF): Multiplying Sequencing Speed and Sensitivity by Synchronized Scans in a Trapped Ion Mobility Device. Florian Meier, Scarlet Beck, Niklas Grassl, Markus Lubeck, Melvin A. Park, Oliver Raether, and Matthias Mann. J. Proteome Res., 2015, 14 (12), pp 5378–5387; Demichev, V., Szyrwiel, L., Yu, F. et al. dia-PASEF data analysis using FragPipe and DIA-NN for deep proteomics of low sample amounts. Nat Commun 13, 3944 (2022).

Note 2: includes protein extraction from 10 tissue samples (5 control, 5 treated), protein quantitation and trypsin digestion, peptide purification using ZIP tips, 90min DDA-PASEF (Data-Dependent Acquisition) LCMS+MS/MS gradient on Bruker timsTOF-PRO, and quantitative data analyses using PEAKS software.
Client processed Biological Quintuplet

$1,2213

Note 3: Center provides reagents for protein extraction, quantitation, and trypsin digestion.  Client performs all sample processing and provides purified (C18 tip processed) peptides. Center acquires DDA-PASEF on timsTOF-PRO. Client completes all the data analyses (following training).