Rates are for University of Missouri users. External users should contact Associate Director Brian Mooney.
|Gel band or spot (coomassie-stained)||$192 (digest + QTOF/Orbitrap LC-MS/MS)|
|20 bands/spots by LCMS/MS||$2,860 (includes spot excision)|
|1 band/spot client-processed (with Center reagents)||$97 (Digest + LC-MS/MS)|
|Protein in solution (e.g. recombinant protein or complex lysate)||$96.70 (digest + large-format ZIP tip + Bruker timsTOF LC-MS/MS)
High-Coverage Global Proteomics
Bruker timsTOF-PRO with PASEF1
|Differential Protein Abundance analyses of a Biological Quintuplet2||$2,796 (Identification and Quantitation of >2000 proteins)
$3,530 (Data-Independent Acquisition, ID & Quant of >3000 proteins)
Note 1: Parallel Accumulation-Serial Fragmentation (PASEF): Multiplying Sequencing Speed and Sensitivity by Synchronized Scans in a Trapped Ion Mobility Device. Florian Meier, Scarlet Beck, Niklas Grassl, Markus Lubeck, Melvin A. Park, Oliver Raether, and Matthias Mann. J. Proteome Res., 2015, 14 (12), pp 5378–5387; Demichev, V., Szyrwiel, L., Yu, F. et al. dia-PASEF data analysis using FragPipe and DIA-NN for deep proteomics of low sample amounts. Nat Commun 13, 3944 (2022).
Note 2: includes protein extraction from 10 tissue samples (5 control, 5 treated), protein quantitation and trypsin digestion, peptide purification using ZIP tips, 90min DDA-PASEF (Data-Dependent Acquisition) LCMS+MS/MS gradient on Bruker timsTOF-PRO, and quantitative data analyses using PEAKS software.
|Client processed Biological Quintuplet||
Note 3: Center provides reagents for protein extraction, quantitation, and trypsin digestion. Client performs all sample processing and provides purified (C18 tip processed) peptides. Center acquires DDA-PASEF on timsTOF-PRO. Client completes all the data analyses (following training).