Reservations

To reserve instrument time you must be a registered user within the EMC's online calendar system, Facility Online Manager (FOM). To register see our policies and procedures page for instrument reservation and service request procedures. If you need additional assistance, please contact us.

Instrumentation

 

  • JEOL JEM-1400

    • This high performance, high contrast TEM is great for viewing biological samples, nanoparticles, and polymers.

      View availability  |  Reserve JEOL

  • ThermoScientific Titan Krios G4 Cryo-TEM (coming soon)

    • “Gold standard” cryo-TEM platform delivers high-throughput biological structure determination at sub-2.0 Å resolution. Advanced cryo-electron tomography delivers 3D modeling of cryogenically preserved cellular ultrastructure.

  • ThermoScientific Spectra 300 STEM  (coming soon)

    • World-class aberration-corrected (S)TEM capable of atomic resolution imaging and spectroscopy.

 

 

  • FEI Quanta 600F Environmental SEM

    • Versatile system capable of analyzing nonconductive media.  It is equipped with a Bruker EDS system which provides extremely high throughput and improved light element detection, as well as a Protochips Fusion platform for in-situ electrothermal measurements.

      View availability  |  Reserve Quanta

  • FEI Scios DualBeam

    • The combination of focused ion beam (FIB) and scanning electron microscopy (SEM) techniques allows site-specific analysis and precise ablation of materials on the micro scale.  Equipped with EBSD for crystallographic microstructure characterization.

      View availability  |  Reserve Scios

  • ThermoScientific VolumeScope 2

  • ThermoScientific Helios Hydra UX DualBeam (coming soon)

    • Variable plasma sources facilitate greatly improved FIB milling rates over traditional Ga+ systems.  Incorporated TOF-SIMS allows for ppb-level chemical species identification at 50 nm spatial resolution.

  • ThermoScientific Aquilos 2 DualBeam (coming soon)

    • Cryo-FIB system capable of automated production of site-specific lamellas for high-resolution cryo-electron tomography.

  • ThermoScientific Phenom ProX (coming soon)

    • Desktop SEM with EDS that allows for rapid sample screening and routine analysis.

 

 

  • Wohlwend High Pressure Freezer
    Rapid cryoimmobilization of biological specimen has many advantages over traditional processing methods. The HPF Compact 02 sets a new standard in high pressure freezing for biological samples up to 0.2 mm thickness and 0.6 mm³ volume. Its robust, reliable and tested design offers excellent cryofixation quality with great reproducibility.

  • E.A. Fischione Model 1070 Nanoclean Plasma Cleaner — With the ability to control ratios of 3 different gases (Argon, Oxygen and Hydrogen), flow rates and time, the Model 1070 Nanoclean Plasma cleaner is ideal for cleaning residual hydrocarbons off TEM holders and samples, facilitating better analytical spectroscopy and imaging, as well as making carbon films on TEM grid hydrophilic or selectively thinning the films. Nanoclean 1070 User Manual (PDF)

    Reserve Plasma Cleaner

  • Pelco Easiglow Glow Discharge — The Pelco Easiglow was a generous gift from Endowed Chair Linda Randall and has allowed University of Missouri investigators the ability to pursue protein structural analysis via negative staining and cryo-EM. The Easiglow temporarily alters the charge on a carbon film — this charge can be selected by altering the composition of the gas molecules in the chamber of the unit.

    Reserve Glow Discharger
     
  • EMS 150TES Sputter Coater — The EMC utilizes most commonly a platinum target to sputter a thin conductive layer on specimens that are imaged under the high vacuum of a scanning electron microscope.  Targets available include: Pt, Au, Cr, Ta, Ti, Ni.  Please see our Sputter Coater Quick Guide (PDF) for simple operation. Q150TES Instruction Manual

    Reserve Sputter Coater
     
  • Emitech K950 Carbon Evaporator — For specimens that need a conductive layer for high vacuum SEM imaging, utilizing this instrument a thin (15 nm) carbon layer can be applied without interfering with X-ray energy dispersive spectroscopy or identification of elemental composition via backscattered electrons. Carbon evaporator instructions (PDF)

    Reserve Carbon Evaporator
     
  • FEI Mark IV Vitrobot — The Mark IV Vitrobot is an automated vitrification robot to form thin vitreous films of ice < 1 micron in width on holey carbon films. Parameters to vary include volume, humidity, temperature, blot force, blot time, buffer salt, buffer pH, sample concentration. FEI Mark IV Vitrobot (WMV)

    Reserve Vitrobot
     
  • Gatan Ultrasonic Disc Cutter (Model 601) — For preparation of 3mm disc to fit into a TEM holder. Gatan Ultrasonic Disc Cutter (Model 601) user's guide (PDF)
     
  • Gatan Dimpler (Model 656) — This instrument takes a 3mm disc created with the Model 601 and dimples the specimen to assist with creation of an electron transparent specimen. Gatan Dimpler user's guide (PDF)
     
  • Gatan Precision Ion Polishing System (Model 691) — Using two argon ion sources, a 3 mm disc is successively abraded under vacuum to create an electron-transparent specimen. Gatan Precision Ion Polishing System user's guide (PDF)
     
  • Buehler Four-Stage Grinding and Two-stage polishing table — Preparation of polished bulk specimens (PDF)

    Reserve polishing table
     
  • Buehler Low Speed Diamond Saw — Diamond saw instructions (PDF)
     
  • Pelco Biowave — During traditional biological sample preparations, water within specimens is replaced by resin via dehydration. These solution exchanges take considerable time. Microwaves have been employed to facilitate faster solution exchange and are utilized for both TEM and SEM biological sample preparation. Pelco Biowave manual (PDF)
     
  • Tousimis AutoSamdri 815 — AutoSamdri 815 details (PDF)
     
  • Leica Ultracut UCT Microtomes & FCS cryo-attachment
     
  • Leica EM UC7 Ultramicrotome
     
  • Leica EM Automated Freeze Substitution Device
     
  • Leica KMR3 Knifemaker — Leica EM KMR3 operating manual (PDF)
     
  • Spin Coater — Spin coater with 100 programmable settings. Chucks (1″, 1/2″ and 3/16″ vacuum spin chucks) for substrate sizes from 10 mm – 200 mm, silicon wafers, PMMA & developer available. Spin Coater manual (PDF)

 

 

Funding Acknowledgements

Funding for EMC instrumentation has been supported by multiple sources including several grants from the National Science FoundationNational Institutes of Health, University of Missouri’s Program for Research Infrastructure and Matching Expenses (PRIME) and different departmental Research Incentive Funds (RIF).