Microscale Thermophoresis
The MIC houses a state-of-the-art thermophoresis instrument (Monolith X) for determining the protein-ligand and protein-protein affinity. This instrument measures change in thermophoresis which depends on the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. Advantages of this instrument include low sample consumption (ca. 100 microliters of low micromolar protein samples) and speed of measurement (under one hour for 16-point titration curve).
To learn more about Monolith X and setting up binding affinity measurements, use the protocol (link below).
Monolith X user manual
Additional details and training
For additional details and to schedule a training session for using these machines, please contact us.