Monolith NT.115

The MIC houses a state-of-the-art thermophoresis instrument (Monolith NT.115) for determining the protein-ligand and protein-protein affinity. This instrument measures change in thermophoresis which depends on the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. Advantages of this instrument include low sample consumption (ca. 100 microliters of low micromolar protein samples) and speed of measurement (under one hour for 16-point titration curve).

To learn more about Monolith NT.115 and setting up binding affinity measurements, use the protocol (link below).

Monolity NT.115 manual nano-temper MST starting guide

Biacore T100

Biacore T100 is a high-performance system for real-time biomolecular interaction analysis. Biacore T100 uses surface plasmon resonance technology to determine the binding affinity between two proteins or between protein and ligand.

Details coming after Feb. 14, 2023.

Additional details and training

For additional details and to schedule a training session for using these machines, please contact us.