Protein Solutions DynaPro MSTC (temperature-controlled, micro-sampler)

The DynaPro-MS (MicroSampler) is an ultra-sensitive instrument combining 45 µl sample volumes with the highest sensitivity available over the 0.5 nm - 200 nm radius size range. The DynaPro is highly sensitive in the detection of aggregation — even trace amounts — which often go undetected with other techniques such as gel filtration chromatography. It measures the hydrodynamic radius down to 1.0nm and provides the distribution independent of solvent conditions so that the macromolecular behavior may be studied in virtually any solution condition. Measurements take only a few minutes and samples are generally recoverable.

For questions about getting started with a Dynamic Light Scattering project contact Dr. Kamal Singh (singhka@missouri.edu) at 573-882-9024 or 573-884-1281.

Dynamic Light Scattering Protocol

Protocol for DynaPro99 Molecular Size Instrument (Dynamic Light Scattering)

  1. Turn on the measurement unit, then turn on the power unit, let it warm up for several minutes. Start the Dynamics program on the computer (icon on desktop).
  2. Clean the cuvettes before and after use with water and ethanol; dry the cell using "air-it." (It is a good idea to fill a cuvette with water and check for light scattering before testing your sample. If scattering is present, then the cuvette is not clean).
  3. Filter your sample whenever possible before applying it to the filter-kit. Unscrew to open the microfilter, add a 0.2µm filter membrane in between the 2 halves, and screw the microfilter tight. Rinse the microfilter with water to make sure there is no leakage.
  4. To get your sample into the syringe, filter it through the microfilter. Remember there will be about 40µl deal volume inside the filter.
  5. Inject the sample into the cuvette, but do not over fill. Put the cuvette frosty side left into the microsampler, close the lid. Wait for 10 or 20 mins.
  6. On the computer program, go to "Tools" menu for experiment settings and optics configuration, based on what your experiment runs, you can adjust these parameters for best performance later.
  7. Push start in the count rate window, monitor count rate, wait until it becomes stable, then stop it.
  8. Push start in the measurement window, collect at least 10 stable data points. Go to .analysis. menu for data collection. Save/print your data.
  9. Turn off the machine, log your time of operation in the logbook.