Cell line propagation & maintenance
Cost includes:
- Thawing, expansion, and freezing of cell lines
- Harvesting of the number of cells that the investigator requests
- Histopaquing of cells to separate live from dead (as needed)
Cell line acquisition — discount varies
Cost includes:
- Purchase of ATCC cell lines at a discount to the investigator
- Purchase of BSL-2 and lower
- Shipping and handling charges apply but the costs will be split between investigators when multiple orders are placed at the same time
Liquid nitrogen storage (LN2)
$225 / year
Cost includes:
- Storage of up to 100 vials at -190oC
- Only certain vials are compatible with the LN2 storage system. Please inquire for more details.
Mycoplasma testing
$70/sample for 1-3 samples, $45/sample for more than 3 samples
Cost includes:
Testing of the four most common mycoplasma and acholeplasma species using a luciferase-based method:
- M. arginini
- M. hyorhinis
- A. laidlawii
- M. orale
*Investigator should provide at least 2 ml of culture supernatant from a confluent culture
Monoclonal antibody production
Culture Supernatant:
- $0.50 per ml of monoclonal antibody supernatant (approximately 10-30 ug/ml)
CL-1000 Integra Bioreactor:
- Highly concentrated (0.5-1.5 mg/ml)* antibody supernatant, free of serum and host immunoglobulin
*Concentrations vary depending on the hybridoma
The price per mL varies depending on the quantity of supernatant ordered. The price begins at $65 per mL and decreases as the volume of the order increases. Charges include all supplies and labor. Please inquire for more details.
Tissue culture links
- Tissue culture protocols:
- FBS heat inactive protocol