Equipment & Resources
Leica TCS SP8 MP inverted spectral confocal microscope with tunable white light laser (470-670 nm), fixed visible laser lines (405, 458, 476, 488, 496, 514 nm), resonant scanner, 3 HyD and 2 PMT detectors, and Mai Tai DeepSee multiphoton laser tunable to 680-1060 nm for deep tissue imaging. The system is also equipped with a two-channel time-correlated single-photon counting unit from PicoQuant and two non-descanned HyD-RLD detectors for fluorescence lifetime imaging microscopy (FLIM). The system is capable of spectroscopy imaging, image tiling, and time-lapse. Equipped with environmental box for live cell imaging. In addition, the microscope has been upgraded with a STED super-resolution 3X system that can achieve resolutions of 50 nm or better. Three depletion lasers (592, 660 and 775 nm) enable multicolor detection of a wide range of fluorophores within the STED system. LightGate technology allows gated emission detection (gated STED) for further improved resolution.
The Leica GSD 3D super-resolution microscope is equipped with 405, 488, 560, and 642 nm lasers for multicolor detection of up to 3 fluorophores and with a fast, highly sensitive Andor iXon Ultra 897 EMCCD camera. The system can achieve a resolution of down to 20 nm in the lateral plane and 50 nm in the axial plane. The microscope also includes a fully automated TIRF system, allowing super-resolution to be combined with TIRF microscopy.
The Leica TCS SP8 MP inverted spectral confocal microscope is equipped with powerful diode lasers of 405, 488, 552, and 638 nm and one HyD and two PMT detectors for conventional confocal imaging. The system is also equipped with a digital light-sheet module with a Hamamatsu Orca-Flash4.0 Digital CMOS camera for illuminating samples (e.g., zebrafish embryos) with a thin plane of light and rapidly acquiring multiple images (>100 frames/s) with minimal photobleaching and subsequent 3D-rendering.
Capable of brightfield, DIC, and multi-channel fluorescence. The camera selection includes a Leica DFC290 color camera. Equipped with an environmental chamber with temperature and CO2 control for extended imaging of live cells. Automated image acquisition of a defined region ("tile scanning") can be performed to generate composite images of large samples.
Capable of brightfield and multi-channel fluorescence. Equipped with a highly sensitive monochrome ORCA-ER camera. Equipped with an environmental chamber with temperature and CO2 control for extended imaging of live cells. Automated image acquisition of a defined region ("tile scanning") can be performed to generate composite images of large samples.
Capable of brightfield, darkfield, and DIC/Nomarski. Equipped with a 3-megapixel color CMOS camera.
Capable of brightfield, multi-channel fluorescence, and Rotterman's Relief Contrast. System includes a motorized focus drive that allows for the acquisition of "z-stacks" which can then be used to create an extended depth of focus image where all planes of the image are in focus.
Automated fluorescence, brightfield, phase-contrast, and DIC optics. Equipped with a 1.4-megapixel, 12-bit, cooled QImaging CCD camera.
Capable of widefield fluorescence and brightfield. Equipped with an Optronics 12-bit color CCD camera.
Microdissection/capture of small regions from tissue samples using UV/IR lasers. Capable of brightfield, phase contrast, DIC/Nomarski, and fluorescence with Nikon Eclipse TE2000 inverted microscope. Equipped with a color CCD camera for image and movie acquisition.
Huygens Professional: Rapid 2D and 3D deconvolution of confocal and STED images.
Metamorph: image analysis, object counting, morphological analysis, thresholding, 3D reconstruction/rotation of confocal optical sections.
Bitplane Imaris: 3D reconstruction of optical sections, measurements in 3D, isosurfacing, animations, colocalization analysis.
Adobe Photoshop CS6 Extended: image processing and editing with batch mode.
Leica LAS X 3D Analysis and Measurements: 3D multichannel analysis, measurements and object classification.
Fiji/ImageJ: image processing and analysis.
Thermo Scientific HM525 NX Cryostat for cutting 1-100 micron-thick tissue sections of frozen samples.
Reichert Ultracut S Ultramicrotome for cutting semi-thin & thin plastic sections for light and electron microscopy.
Paraffin Microtome for cutting 1-50 micron thick paraffin sections.
Vibratome 3000 Plus for cutting variable thicknesses as thin as 100 µm of live or fixed specimens with a maximum specimen size of 60x40x15mm.
The Cytology Core has an on-site Life Technologies Supply Center stocked with fluorescent secondary antibodies and markers. Consumables for laser capture microdissection are also available. In addition to our normal inventory, clients may special order anything from the Life Technologies catalog without shipping fees .
The Cytology Core has a server that can be remotely accessed from any computer on the MU campus. It serves as a TEMPORARY holding place for files to facilitate transfer of the data you generate at the Core to a local computer in your lab/office. Please note that due to space limitations the storage space is cleared every morning at 8:00 am, such that your files will only be stored for a maximum of 24 hours and you need to transfer them to your computer on the same day they were placed on the server. For instructions on connecting to this utility contact MCC staff.